Color Control Factor

Small interfering RNA (siRNA) targeting tyrosinase, the key rate-limiting enzyme in melanin biosynthesis. After entering melanocytes, its double-stranded structure dissociates. The antisense strand recruits the RNA-induced silencing complex (RISC) and guides the RISC/siRNA antisense-strand complex to specifically bind to the target sequence region of tyrosinase mRNA via base complementary pairing. Subsequently, under the RNA interference (RNAi) pathway, tyrosinase mRNA is selectively degraded, blocking melanin biosynthesis at its source

Composition Information

Mechanism of Action

1. After siRNA enters melanocytes, its double strands dissociate, and the antisense strand recruits RISC silencing complex.
2. It specifically binds to target sequences of tyrosinase mRNA via base complementary pairing and mediates mRNA degradation.
3. It downregulates tyrosinase gene expression, reduces the activity of tyrosinase protein and decreases pigment biosynthesis.
4. With peptide nanoparticle carrier structure, it penetrates the epidermal stratum corneum and cell membrane to deliver siRNA into cells for efficacy.

Experimental Data

1. Tyrosinase mRNA interference efficiency: 82.81% at 24 h post-transfection; 76.19% at 48 h; 75.46% at 72 h.
2. Tyrosinase protein expression inhibition test: 1.2% pigment-regulating factor reduces tyrosinase protein expression by 83.3%.
3. Cellular melanin inhibition test: TYR‑siRNA suppresses L-tyrosine-induced intracellular melanin accumulation, with melanin inhibition rate of 81.8%.