Dual-Biotic Soothing Extract

Dual-Biotic Soothing Extract is carefully developed by National Cheng Kung University (Taiwan). Customized for sensitive skin, it functions by precisely regulating skin microbiome, balancing skin immunity and strengthening the skin barrier. It efficiently delivers multiple skincare benefits including bacteriostasis, immune balance, anti-inflammatory soothing, barrier repair and antioxidation. Its efficacy is verified by scientific experiments with clear specifications and reliable safety profile. It perfectly meets skincare demands of sensitive skin and interprets a new philosophy of gentle skin care.

Composition Information

Mechanism of Action

1. Microbiome regulation pathway: Inhibit the proliferation of harmful microorganisms via nutrient competition, increasing bacterial outer membrane permeability, altering intracellular osmotic pressure and suppressing macromolecular synthesis.
2. Immune homeostasis pathway: Regulate the differentiation of CD4⁺T cells, relieve excessive activation of Th1 and Th2, maintain Th1/Th2 balance and soothe skin hyperreactivity.
3. Soothing pathway: Downregulate inflammatory factors including TNF‑α, IL‑1, IL‑6 and IL‑8; reduce NO release and inhibit hyaluronidase activity to mitigate inflammatory response.
4. Barrier repair pathway: Upregulate the expression of type Ⅰ collagen (COL‑Ⅰ) and tight junction protein Claudin‑1 to strengthen skin barrier structure.
5. Antioxidant pathway: Scavenge DPPH, hydroxyl radicals and superoxide anion radicals to alleviate oxidative skin damage.

Experimental Data

1. *Staphylococcus aureus* biofilm inhibition test: 3% Dual-Biotic Soothing Extract achieves 80% inhibition against *Staphylococcus aureus* biofilm.
2. Th1/Th2 immune balance test: 2% Dual-Biotic Soothing Extract raises IFN‑γ/IL‑4 ratio by approximately 83.3% and regulates allergy-related immune shift.
3. Inflammatory cytokine inhibition test on HaCaT cells: With 5% Dual-Biotic Soothing Extract, the expression of TNF‑α, IL‑1, IL‑6 and IL‑8 decreases by 88.9%, 87.5%, 91.7% and 83.3% respectively.
4. NO release inhibition test on RAW264.7 cells: 4% Dual-Biotic Soothing Extract reduces NO content by around 70.45% compared with LPS group.
5. Hyaluronidase inhibition test: 4% Dual-Biotic Soothing Extract shows approximately 79% inhibition on hyaluronidase.
6. COL‑Ⅰ assay on fibroblasts: 2% Dual-Biotic Soothing Extract increases COL‑Ⅰ content by 200%.
7. Claudin‑1 assay on keratinocytes: 4% Dual-Biotic Soothing Extract increases Claudin‑1 content by 200%.
8. Free radical scavenging test (5% Dual-Biotic Soothing Extract): DPPH scavenging rate 96.57%; hydroxyl radical scavenging rate 53.77%; superoxide anion radical scavenging rate 34.71%.
9. Human VISIA redness test: Formulation with 5% Dual-Biotic Soothing Extract improves facial flushing and redness within 12–48 h.